Science Behind
In 1998, the Nobel Prize in Physiology and Medicine was awarded to Dr. Robert F. Furchgott, Louis J. Ignarro, and Ferid Murad for their groundbreaking discoveries on nitric oxide as a signaling molecule in the cardiovascular system.
At NoxShot, our products are built on a foundation of rigorous scientific research and experimentation. Our dedicated scientific team conducts extensive studies to explore the benefits and mechanisms of nitric oxide.
Each article here highlights the science behind NoxShot supplements, supporting your wellness journey with trusted, peer-reviewed research.
The Influence of Amaranth (Amaranthus Hypochondriacus) Dietary Nitrates on the Aerobic Capacity of Physically Active Young Persons
Recent evidence indicates that elevating plasma nitrites through dietary nitrates (NO3-) supplementation is associated with enhanced muscle efficiency, fatigue resistance and performance. Beetroot (in various forms) is the dominant source of dietary NO3- primarily due to its vast availability and the simple form of preparation suitable for final consumption. After a few years of research and experimentation, our scientific team identified alternative source rich with dietary NO3- as possible nitric oxide precursor, amaranth (Amaranthus hypochondriacus) with a standardized concentration 9-11% of NO3-. This study aimed to evaluate the effect of single-dose (±400 mg of dietary NO3-) and long-term (6 days) supplementation of amaranth concentrate derived dietary NO3- on aerobic capacity in physically active young people.
Impact of Dietary Potassium Nitrate on the Life Span of Drosophila Melanogaster
The recently defined and yet rather new topic of healthy aging is attracting more attention worldwide. As the world population is getting older, it is rapidly becoming essential to develop and maintain functional abilities at older age and develop mechanisms to protect the senior population from chronic diseases. One of the most effective components, as well as processes associated with aging, is the recently discovered and Nobel prize-awarded—nitric oxide (NO) (as a signaling molecule), which, followed by later discoveries, showed to have a positive metabolic, immunological, and anti-inflammatory effect. Nitrates are one of the most debated topics of the last decade in the scientific community due to their pathways involved in the production of nitric oxide. Thus, the objective of this study is to evaluate the effect of different potassium nitrate concentrate supplementation on Drosophila melanogaster longevity imitating a human carbohydrate-based diet with relationship to possible cause of oxidative stress. Influence of 0.5–3% potassium nitrate medium on the lifespan and motor function in different groups consisting of 100 fruit fly females in each was analyzed. In this assay, female fly species supplemented with potassium nitrate diet showed life span increase by 18.6% and 5.1% with 1% and 2% KNO3, respectively, with a positive impact on locomotor function. In conclusion, we found that low concentration of potassium nitrate medium increased lifespan and locomotor function in Drosophila melanogaster.
Effects of Life-Long Supplementation of Potassium Nitrate on Male Mice Longevity and Organs Pathology
Many short-term studies with dietary nitrate supplementation in humans and animal models reported positive effects on the cardiovascular system, exercise efficiency, and immune function. However, there has been long-standing concern related to cancer and adverse hormonal effects. We studied the long-term effects of different potassium nitrate (KNO3) concentrations on laboratory mice longevity and structural changes in their organs. Four groups of male mice were treated with 0 mg (0%), 45 mg (1%), 90 mg (2%), and 140 mg (3%) KNO3 in the drinking water. The groups were monitored for agility and health status daily. The lifespan of mice and organ pathological changes were analyzed. We found no detrimental effects of life-long supplementation of KNO3 on the survival of mice in treatment groups. Nitrate supplementation was associated with a lower level of pathological changes (p = 0.002). We conclude that KNO3 supplementation had no carcinogenic effect on mice and possibly prevented the organs from aging.
The Effect of Potassium Nitrate Supplementation on the Force and Properties of Extensor digitorum longus (EDL) Muscles in Mice
Adding potassium nitrate (KNO3) to the diet improves the physiological properties of mammalian muscles (rebuilds weakened muscle, improves structure and functionality). The aim of this study was to investigate the effect of KNO3 supplementation in a mouse model. BALB/c mice were fed a KNO3 diet for three weeks, followed by a normal diet without nitrates. After the feeding period, the Extensor digitorum longus (EDL) muscle was evaluated ex vivo for contraction force and fatigue. To evaluate the possible pathological changes, the histology of EDL tissues was performed in control and KNO3-fed groups after 21 days. The histological analysis showed an absence of negative effects in EDL muscles. We also analyzed 15 biochemical blood parameters. After 21 days of KNO3 supplementation, the EDL mass was, on average, 13% larger in the experimental group compared to the controls (p < 0.05). The muscle-specific force increased by 38% in comparison with the control group (p
Athlete's Preparation for Competition with NoxShot Supplement Routine
Before a major competition, the athlete incorporated NoxShot into a demanding training regimen, targeting enhanced performance. The results demonstrated significant improvements in key physiological metrics and notable achievements.
